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NCI CANCERLIT^® Search: Retinoblastoma Gene (RB) - February 2002

National Cancer Institute^®
Last Modified: February 1, 2002

[Study of APC, Rb, c-met gene copy numbers of human gastric mucosa epithelial cell line GES-1]

Primed in situ labeling (PRINS) for evaluation of gene deletions in cancer.

DNA typing of hair shafts by microwave irradiation: real or deceptive evidence?

Table of Contents

1
UI - 11776612
AU - Zheng S; Ke Y
TI - [Study of APC, Rb, c-met gene copy numbers of human gastric mucosa epithelial cell line GES-1]
SO - Zhonghua Zhong Liu Za Zhi 1999 Nov;21(6):409-11
AD - Beijing Institute for Cancer Research, School of Oncology, Beijing Medical University, Beijing 100034.
OBJECTIVE: To study the copy number of oncogene/tumor suppressor genes in GES-1 cell line to identify its characteristics. METHODS: Bio-14-dATP was incorporated into APC, Rb, c-met gene cloned in plasmid by nick translation. Fluorescence in situ hybridization (FISH) of interphase nuclei of GES-1 cells was performed. RESULTS: Two copies of APC were shown in 48% interphase nuclei and 3 copies in 22%; 71% and 80% cells had normal copies of Rb and c-met genes in their nuclei, respectively. CONCLUSION: GES-1 cell line is a relatively normal gastric mucosa epithelial cell line and can be used as a human in vitro model system for the study of carcinogenesis.

2
UI - 11807886
AU - Tharapel SA; Kadandale JS
TI - Primed in situ labeling (PRINS) for evaluation of gene deletions in cancer.
SO - Am J Med Genet 2002 Jan 15;107(2):123-6
AD - Cytogenetics Reference Laboratory, Pathology and Laboratory Medicine Service, V.A. Medical Center, Tennessee 38104, USA. sugandhi.tharapel@med.va.gov
Rearrangements involving the 13q14 and 17p13 chromosomal regions are often observed in leukemias and lymphomas. These rearrangements are not always identifiable cytogenetically. In more than 50% of cases, deletions occur at the submicroscopic level and the karyotypes appear normal. Molecular cytogenetic techniques such as fluorescence in situ hybridization (FISH) have accordingly contributed to the identification of a variety of subtle rearrangements such as those involving submicroscopic deletions. However, FISH is expensive, time consuming, technically burdensome, and requires cloned DNA probes. A newer technique, primed in situ labeling (PRINS), has been tested as a possible alternative to FISH. To assess the utility and efficiency of the PRINS method in the detection of RB1 and p53 deletions, we evaluated 10 patients with hematological disorders and known rearrangements, i.e., deletions involving 13q14 and 17p13 regions. The data in these cases were validated against data obtained with standard FISH probes. Our results indicate that PRINS could be used with relative ease in cytogenetics laboratories and could serve as an alternative to conventional FISH for defining deletions involving unique sequences. Copyright 2001 Wiley-Liss, Inc.

3
UI - 11724429
AU - Ferri G; Colalongo C; Bini C; Pelotti S; Pappalardo G
TI - DNA typing of hair shafts by microwave irradiation: real or deceptive evidence?
SO - Int J Legal Med 2001 Oct;115(2):118-20

The above citations and abstracts reflect those newly added to CANCERLIT for the month and topic listed in the title. The citations have been retrieved from CANCERLIT using a predefined search strategy of indexed subject terms. Although the search strategy has been refined as best as possible, citations may appear that are not directly related to the topic, and occasionally relevant references may be omitted.

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